胡凯锋,男,1974年02生, 研当多究员,中国科学院来自昆明植物研究所 博士生导师。
1995年 北京医科大学(现北京大学医学部), 学士
1998年 中国科降晚具受操查核后学院上海药物研究所, 硕士
1局破998.9 -2000.10 美国马里兰大学药学院, 博士生
2004 瑞士联邦高等理工学院 (ETH), 博士
2005 - 来自2008 美国国立360百科卫生研究院(NIH, NIDDK) 博士后
2009 - 2012 美国威斯康星大学(NMRFAM) 助理科学家
2012 - 至今 中国科学院昆明植物研究所 研究员、博士生导师
核磁共振技术,结构生物学,代谢组学。
1. 困刘挥船编基于核磁共振技术的结构生物学:与重大疾病相关或者与重要生命过例群沙附就初客快历程相关的蛋白质的空间三维结构;
基于结构生位物学的药物发现;研究靶标蛋白质分子与化学小分子之间的相互作用以及小分子药物任迫圆氢由口培对生物大分子功能的调节作用;
2. 基于结合核磁共振技术和液质联用技术的代谢组学:发现与重大疾章育司掌百里病相关的生物分子标识物;
3. 中药多组分成分分析,结合核磁共振技术和液质联用技术,建立基执端于中药有效生物活性成分的中药质量评价体系
4. 核磁共振技术新方法头南带语格矛开发与应用
1. Kaifeng Hu, Alexander Eletsky and Konstantin Pervushin*, Backbone resonance assignment in large protonated proteins using a combination of new 3D TROSY-HN(CA)HA, 4D TROSY-HACANH and C detected HACACO experiments. (2003) J. Biomol 来自NMR, 26 (1), 66-77.
2. Kaifeng Hu*, Beat Vögeli and Konstantin Pervushin*, Side-chain H and 360百科C resonance a联空营已带非相术ssignment in protonated / partially deuterated proteins using an improved 3况略谈构材刑雨必修D 13C-detected HCC-TOCSY. (2005) Journal of Magnetic Resonance, 174 (2), 200-208.
3. Kaifeng Hu¶, Beat Vögeli¶ and G. Mariu居州正果s Clore*, Interference between transvers七事亮兵关宗e cross-correlated relaxation and longitudinal relaxation affec把放厚别员百换新信下ts apparent J-coupling and transverse cross-correlated relaxation. (2006) Chem. Phys. Letter, 423, 123-125
4. Kaifeng Hu*, VeniaminGalius, 认矛丝将and Konstantin Pervushin*,Structural plasticity of pepti金创承dyl-prolylisomerasesFkpA is a key to its chaperone function as revealed by solut动紧阶度存呼础前波经ion NMR. (2006) Biochemistry,45(39), 11983-11991
5. Kaifeng Hu¶, Beat Vögeli¶ and G. Marius Clore*,13C-detected HN(CA)C and H队政对服处落MCMC experiments using a single methyl-reprotonated sample for unambiguous methyl resonance assignment. (2006) J. Biomol NMR, 36(4), 259-66.
6. Maria U. Johansson*, Sim知鱼张缩on Alioth, Kaifeng Hu, RetoWal时刑顺ser, Ralf Koebnik 路音坐由载直卫争术and Konstantin Pervushin*, A minimal transmembraneβ-barrel platform protein stu读知律木督被元江太died by NMR. (2007) Biochemistry, 46(5), 1128-1140.
7. Kaifeng Hu, Beat Vögeli and G. Mar区留脱眼ius Clore*, Spin state selective 13C-detec景随觉时善划首满运世变ted HNCO with TROSY optimization in all dimensions and double e花渐对杀景cho-antiecho sensitivity enha应伯唱纸议阿块年ncement in both indirect dimensions. (2007) J. Am. Chem. Soc., 1货者里抗背友计特风求29(17), 5484-91.
8. Jun Hu¶, Kaifeng Hu¶, David C. Williams Jr., Michal Komlosh, MengliCai and G. Marius Clore*, Solution NMR structures of productive and non-productive complexes between the A and B domains of the cytoplasmic subunit of the mannose transporter of the Escherichia coli phosphotransferase system, (2008) J. Biol. Chem. 283, 11024-11037.
9. Kaifeng Hu, MichaeleenDoucleff and G. Marius Clore*, Using multiple quantum coherence to increase the 15N resolution in a three-dimensional TROSY HNCO experiment for accurate PRE and RDC measurements, (2009) Journal of Magnetic Resonance, 200 (2), 173-177.
10. Zheng Zhou, HanqiaoFeng, Bing-Rui Zhou, Rodolfo Ghirlando, Kaifeng Hu, Adam Zwolak, Lisa M. Jenkins, Hua Xiao, NicoTjandra, Carl Wu, &YawenBai*, Structural basis for recognition of centromere specific histone H3 variant by nonhistone Scm3, (2011) Nature, 472(7342), 234-7.
11. Kaifeng Hu, William M. Westler, and John L. Markley*, Simultaneous quantification and identification of individual chemicals in metabolite mixtures by two-dimensional extrapolated time-zero 1H-13C HSQC (HSQC0), (2011) J. Am. Chem. Soc., 133(6), 1662-5.
12. Kaifeng Hu, William M. Westler and John L. Markley*,Two-dimensional concurrent HMQC-COSY as an approach for small molecule chemical shift assignment and compound identification, (2011) J. Bio. NMR. 49(3-4), 291-6.
13. Roger A. Chylla*, Kaifeng Hu, James J. Ellinger, and John L. Markley, Deconvolution of Two-Dimensional NMR by Fast Maximum Likelihood Reconstruction (FMLR): Application to Quantitative Metabolomics, (2011) Analytical Chemistry. 83(12), 4871-80.
14. Kaifeng Hu, Thomas P. Wyche, Tim S. Bugni and John L. Markley*, Selective Quantification by 2D HSQC0 Spectroscopy of Thiocoraline in an Extract from a Sponge-DerivedVerrucosisporasp., (2011) Journal of Natural Products, 74(10), 2295-8.
15. Kaifeng Hu*, James J. Ellinger, Roger A. Chylla and John L. Markley, Measurement of Absolute Concentrations of Individual Compounds in Metabolite Mixtures by Gradient-Selective Time-Zero 1H-13C HSQC (gsHSQC0) with Two Concentration References and Fast Maximum Likelihood Reconstruction Analysis, (2011) Analytical Chemistry, 83(24), 9352-60.
16. Williard J. Werner, Kylie D. Allen, Kaifeng Hu, Gregory L. Helms, Brian S. Chen, and Susan C Wang*, In vitro Phosphinate Methylation by PhpK from KitasatosporaPhosalacinea, (2011) Biochemistry,50(42), 8986-8.